The Effects of Tongmai Tang on the Production of NO and the Balance of Fibrinolysis System in Injured Vascular Endothelial Cells
- VernacularTitle:通脉汤对损伤的血管内皮细胞NO生成及纤溶平衡的影响
- Author:
Fei HE
;
Ming SUN
;
Hongyan ZHOU
- Publication Type:Journal Article
- Keywords:
Tongmai Tang;
Endothelium;
Nitric oxide;
Fibrinolysis
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Tongmai Tang on the production of nitric oxide(NO) and the balance of fibrinolysis system in human umbilical vein endothelial cells(HUVEC) injured by oxidized low density lipoprotein(oxLDL). Methods The herbage-contained serum of Tongmai Tang and oxLDL were prepared, then the human HUVEC-304 cells were divided into 4 groups: ⑴control group; ⑵bovine serum control group; ⑶oxLDL group: 100mg/L oxLDL, and ⑷Tongmai Tang group: 5% herbage-contained serum of Tongmai Tang and 100mg/L oxLDL. After 24h incubation, the morphology of the endothelial cells was observed under the inversion microscope, while the concentrations of NO, t-PA and PAI-1 in the culture medium were measured. Results ⑴The endothelial cells in oxLDL group were seriously injured, while those in Tongmai Tang group got better; ⑵the concentrations of NO and t-PA in the oxLDL group were significantly lower than those in the control group(P
