Human Prolactin Enhances the Production of IgG and Anti-dsDNA Antibody in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus
- VernacularTitle:催乳素对系统性红斑狼疮患者单一核细胞产生IgG和抗dsDNA抗体的影响
- Author:
Feng LI
;
Shufang FENG
;
Jinhua XU
;
Housheng WU
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus, systemic;
Prolactin;
Immunoglobulin G;
Antibodies, antinuclear
- From:
Chinese Journal of Dermatology
1995;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of human prolactin (PRL) on total IgG and anti-dsDNA antibody production in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE). Methods PBMC from SLE patients and control subjects were cultured, with the stimulation of PRL, interleukin 2 (IL-2), interleukin 10 (IL-10) and phytohemagglutinin (PHA). 3H-thymidine (3H-TdR) incorporation assay was used to study the proliferation of PBMC. Total IgG and anti-dsDNA antibodies in the cultured supernatants were measured by enzyme-linked immunosorbent assay (ELISA) in 19 patients of SLE and in 6 control subjects. Results ①The proliferation of PBMC in vitro was enhanced by 10-9 mol/L PRL in 19 patients of SLE. ②The production of IgG and anti-dsDNA antibodies in PBMC from 10 active SLE patients was much higher than that of 9 inactive patients (P
