Helicobacter-specific 16S ribosomal DNA identifided from patients with cholangiocarcinoma
- VernacularTitle:胆管癌组织螺杆菌16S-rRNA基因的检测及意义
- Author:
Huixing CHEN
;
Yanling CHEN
- Publication Type:Journal Article
- Keywords:
Cholangiocarcinoma;
Helicobacter;
Genes
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to determine whether Helicobacter infection was associated with cholangiocarcinomas in human. Methods Resected liver tissues from 11 patients with cholangiocarcinoma were subjected to polymerase chain reaction(PCR) analysis using two sets of Helicobacter-specific 16S ribosomal RNA primers, and each sample was amplified twice. Control group included two cavernous hemangioma cases and four cyst cases. Results The expected 400-bp and 300-bp fragments of Helicobacter 16S rRNA were amplified from 6/11(55%) and 7/11(64%) cholangiocarcinoma samples, 6 samples were both positive for the two sets primers. The 6 control specimens were negative. The difference between the two groups was significant( P
