Mechanism by which cyclosporine A downregulates transcription of interferon-? gene after orthotopic liver transplantation in rats
- VernacularTitle:环孢素A对原位肝移植后干扰素?基因转录的调控机制
- Author:
Aibin ZHANG
;
Shusen ZHENG
;
Changku JIA
;
Yan WANG
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Cyclosporine;
NF kappa B;
Interferon type Ⅱ
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mechanism by which cyclosporine A downregulates transcription of interferon-? gene after murine orthotopic liver transplantation.Methods Orthotopic murine liver transplantation model was employed and rats were divided into 3 groups. GroupⅠ: syngeneic control (Wistar-to-Wistar); GroupⅡ: acute rejection (SD-to-Wistar). GroupⅢ: acute rejection treated with cyclosporine A (SD-to-Wistar+CsA). EMSA was employed to analyze NFAT and NF-?B DNA-binding activity of splenocytes, RT-PCR was employed to analyze IFN-? mRNA transcription intragraft on 1,3,5,7,12 day posttransplant in each group, respectively. Histopathological examination was also performed for reference.Results In comparison to groupⅠ, NFAT and NF-?B DNA-binding activity of splenocytes in groupⅡincreased significantly( P
