Effect of Transforming Growth Factor -?1 on Rat Islet
- VernacularTitle:TGF-1转染对胰岛生物学性状的影响
- Author:
Xiaotao XU
- Publication Type:Journal Article
- Keywords:
Transforming growth factor;
Islet;
Transplantation
- From:
Journal of Chinese Physician
2002;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transforming growth factor ?1 on secretion function of rat islet allograft in vivo and in vitro.Methods Expressing vector of rat TGF-?1 was constructed and transfected to purify Wistar rat islets. Expression of TGF-?1 and rat insulin1 gene (Ins1) in experiment and control group was detected by RT-PCR; Streptozotocin 60mg/kg intra-abdominal injection prepared the SD rat diabetic model. Insulin of cultured islets at basal and stimulated phase in vitro was detected by RIA; Islet allotransplantation was performed under recipient SD rats' renal subcapsule. The level of blood glucose was recorded before and after transplantation. The glucose tolerance was also tested.Results Expression of both TGF-?1 and Ins1 were detected in experimental group, but only Ins1 was detected in control one; Experiment and control islets secreted 3 86?1 24ng/mL and 4 13?1 45ng/mL at basal phase, 8 43?1 59ng/mL and 8 95?1 74ng/mL at stimulating phase, respectively.No significant difference was observed between two groups, but it was in groups. Experimental group' mean normal glycemia value (26 8?2 9) days was significantly longer that control group (13 2?4 5 days). Control group's glucose tolerance curve was significantly above experiment one's.Conclusion TGF-?1 can prolong the survival of islet allograft while it does not impair graft's endocrine function.
