Gene Sequencing Analysis on Vibrio Cholera O_(139) Strains TcpA of Pingxiang Area
- VernacularTitle:萍乡地区O_(139)群霍乱弧菌TcpA基因序列分析
- Author:
Changqi WANG
;
Bingquan YAO
;
Dinshang HE
- Publication Type:Journal Article
- Keywords:
Vibrio cholerae O 139;
Nucleotide sequence;
Polymerase chain recation
- From:
Journal of Chinese Physician
2002;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the TcpA gene sequence and analyze the characteristics of O 139 epidemic strains.Methods PCR was performed to detect the O 139 VC in the bacteria sample.The positive sample was sequenced and analysed.Results PCR products of one strain in the positive sample were sequenced.The gene sequences showed 100% nucleotide homology to O 139 strains adn Eltor strains 96.0%.Conclusion The present study indicates that O 139 VC and O1EVC in Pingxing area are genetically nearest in genetic relation.The study has significance in the diagnose and treatment of Vibrio cholerae in Pingxiang area.