Determination of the Concentration of Propofol in Plasma by RP-HPLC with Fluorescence Detection
- VernacularTitle:反相高效液相色谱-荧光法测定血浆中丙泊酚的浓度
- Author:
Meihua CAI
;
Shanjuan WANG
;
Xiangrui WANG
;
Yannan HANG
- Publication Type:Journal Article
- Keywords:
propofol;
HPLC;
fluoresence detection
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To build up a method for the determination of propofol in plasma by RP-HPLC with fluores?cence detection.METHODS:The separation was performed on a reversed-phase Zorbax Eclipse XDB-C 18 column(150mm?4.6mm,5?m)with a mobile phase consisting of methanol-acetonitrile-0.005mol/L sodium acetate buffer(pH4.0)(55∶20∶25,V/V).Propofol was extracted from plasma and dissolved in the mobile phase then detected at276/310nm.RESULTS:The calibration curve had the fine linearity in the concentration range of0.015625～8?g/ml(r=0.9998).The limit of detection(LOD)was1ng/ml(S/N ratio=3),the limit of quantification(LOQ)was10ng/ml.The absolute recovery was89.33%～93.37%,the relative recovery was97.75%～103.31%.The within-day and between-day precision(RSD%)was1.38%～5.02%and4.45%～9.056%respectively.CONCLUSION:The method is simple,stable and highly sensitive and can meet with the research of clinical pharmacokinetics.
