Determination of Diclofenac Sodium in Huamn Plasma by HPLC
- VernacularTitle:高效液相色谱法测定人血浆中双氯芬酸钠的浓度
- Author:
Haijun MIAO
;
Gaolin LIU
- Publication Type:Journal Article
- Keywords:
diclofenac sodium;
HPLC;
blood concentration of drug
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC procedure to determine diclofenac sodium in human plasma.METHODS:The plasma sample was deproteinized by adding acetonitrile,A Hypersil-BDS(250?4.6mm,5?m)column with a mobile phase consisting of methanol-water-10%phosphate acid(60∶40∶1)was used,the detecting wavelength was276nm,column tem?perature was35℃.RESULTS:The linear range was20.50～2050.0ng/ml(r=0.9998,n=5),the detective limit was10ng/ml.The recovery was99.47%～105.4%with the within-day RSD from3.77%to5.81%(n=5),and the between-day RSD from4.93to8.96%(n=5)at three different concentrations.CONCLUSION:The method is simple,rapid and suitable for the pharmacokinetic study of diclofenac sodium.
