Effects of 2-chloroadenosine and IPP on intracellular reactive oxygen species
- VernacularTitle:2-氯腺苷和异戊烯焦磷酸的细胞内抗氧化作用
- Author:
Jie ZHENG
- Publication Type:Journal Article
- Keywords:
2-chloroadenosine;
Isopentenyl pyrophosphate;
Reactive oxygen species;
Antioxidants
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
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Abstract:
AIM: To study the effect of selective A1 adenosine receptor agonist, 2-chloroadenosine (2-CA), and non-peptide phosphoantigen isopentenyl pyrophosphate (IPP) on the intracellular reactive oxygen species (ROS) at the level of signaling event. METHODS: M1 cell line, derived from a subclone of the SV40-transformed xeroderma pigmentosum (XP) cell line, was treated with 2-CA, 8-br-cAMP, IPP at 37 ℃ incubator for 30 min, and intracellular ROS was measured by flow cytometry and CytoFluorometer. RESULTS: Under an appropriate control, 2-CA and IPP reduced intracellular ROS by 20%-30%. CONCLUSION: The data revealed that 2-CA and IPP inhibited cellular oxidant formation at the level of signaling event, suggesting both may have some anti-oxidant properties in vivo.
