Effect of antifibrotic herb serum on rat hepatic stellate cell proliferation and collagen synthesis
- VernacularTitle:大鼠含抗肝纤维化中药的血清对肝星状细胞的作用
- Author:
Yunbiao LING
;
Ruiyun XU
;
Wanshou QIU
;
Zhonghua CHU
;
Hongzhi YANG
;
Zhi TAN
;
Qingji OU
- Publication Type:Journal Article
- Keywords:
Liver cirrhosis;
Hepatic stellate cells;
Collagen;
Drug, Chinese herbal;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16,8,4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro . Then we mensurated the radioactivity of HSC admixed with [ 3H]proline and [ 3H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0 5, 1, 2 h after intragastrical infusion inhibited HSC proliferation ( P 0 05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action.
