Effect of fibronectin on proliferation and collagen synthesis in cardiac fibroblasts from spontaneously hypertensive rats
- VernacularTitle:纤维粘连蛋白对培养的自发性高血压大鼠心肌成纤维细胞增殖及胶原合成的影响
- Author:
Liangdi XIE
;
Ying HAN
;
Shuilong CHEN
;
Huajun WANG
;
Changsheng XU
- Publication Type:Journal Article
- Keywords:
Myocardium;
Fibroblasts;
Fibronectins;
Proliferation;
Collagen
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of fibronectin (FN) on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts (CFb) derived from SHR (CFb SHR ) and WKY (CFb WKY ). METHODS: CFb derived from 12-week-old spontaneously hypertensive rat (SHR) and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was measured by cell number counting and [ 3H]-TdR incorporation using 24-well plates pre-coated with 5 ?g/cm 2 of FN. Collagen synthesis was determined by [ 3H]-proline incorporation. RESULTS: As compared with control, the cell number of fibroblasts derived from SHR and WKY were significantly increased to 163.75% and 170.42% respectively after 72 h incubation with FN in the presence of 0.4% FCS from a intial cell density of 1?10 4 cells/mL. DNA synthesis of CFb was markedly promoted by FN [CFb SHR : ( 44?734 ? 8?981 )counts/min vs ( 29?233 ? 6?746 ) counts/min, P
