Cloning of P1 and P3 promoters of human insulin-like growth factorⅡ gene
- VernacularTitle:人胰岛素样生长因子Ⅱ基因P1、P3启动子克隆及意义
- Author:
Shaohui TANG
;
Donghua YANG
;
Jianchang SHU
;
Wei HUANG
- Publication Type:Journal Article
- Keywords:
Insulin-like growth factor Ⅱ;
Genes;
Promoter regions (genetics);
Cloning, molecular
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To clone P1 and P3 promoters of the human insulin-like growth factor Ⅱ(IGF-Ⅱ) gene. METHODS: According to the complete DNA sequence of IGF-Ⅱ gene, the nested primer PCR was performed for amplifying P1 and P3 promoter fragments of the gene from human L-02 cell line.These PCR products were analyzed by agarose gel electrophoresis, and cloned by using TOPO TA Cloning kit. The positive clones containing P1 and P3 fragments were selected and confirmed by sequencing.RESULTS: The DNA sequences of P1 and P3 promoters cloned were accordant with GenBank data. CONCLUSION: In this study P1 and P3 promoters of the IGF-II gene were cloned successfully.
