Expression of phosphatidylinositol 3-kinase during the proliferation of the hypoxic rat pulmonary arterial smooth muscle cells
- VernacularTitle:PI-3K在低氧大鼠肺动脉平滑肌细胞增殖中的表达
- Author:
Qilin AO
;
Sheng ZHOU
;
Mi XIONG
;
Dixu WANG
- Publication Type:Journal Article
- Keywords:
1-phosphatidylinositol 3-kinase;
Anoxia;
Pulmonary artery;
Muscle, smooth;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of phosphatidylinositol 3-kinase (PI-3K) during the proliferation of the hypoxic pulmonary arterial smooth muscle cells(PASMC). METHODS: The cultured PASMC was divided into two groups: serum-free medium (SFM) group and 48 hours hypoxia (H48h) group. Immunohistochmistry(IHC) and Flowcytometer(FCM) were used to detect the cultured PASMC. RESULTS: The positive expression of PI-3K p110 existed in both cultured PASMC groups. The staining intensity in H48h group (0 1891?0 0301) was significantly stronger than that in SFM group (0 1025?0 0164, P
