Determination of four bufadienoiides in human liver by HPLC
- VernacularTitle:高效液相色谱法测定肝组织中4种蟾蜍二烯内酯类化合物
- Author:
Zhaohong WANG
;
Xiaoping HOU
;
Yi HE
- Publication Type:Journal Article
- Keywords:
Forensic toxicologicai;
bufadienolides;
HPLC;
SPE
- From:
Chinese Journal of Forensic Medicine
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method used for quantitative determination of bufadienolides in human liver tissues by HPLC. Methods using solid phase extraction of human liver with Oasis (HLB car-tridge coupled with reserved phase HPLC and diode array detection. Results Recoveries obtained from spiked liver for the bufadienolides were better than 70% . The linearity was studied up to 1200ng/g and the detection limits of the method was 0.4ng for cinobufotalin and bufalin, 0.5ng for cinobufagin and resibufo-genin. Conclusion This method is fast and accurate. It is useful for forensic medicine detection.
