Molecular Mechanism of c-jun Antisense Gene Transfection in Protecting Cardiomyocyte from Injury of Hypoxia and Burn Serum Treatment
- VernacularTitle:c-jun反义基因转染对缺氧复合烧伤血清处理心肌细胞保护作用的分子机制研究
- Author:
Angeng HU
;
Yuesheng HUANG
- Publication Type:Journal Article
- Keywords:
PKC;
JNK;
Cardiomyocyte;
Hypoxia;
Burn sera;
c-jun;
Gene transfection
- From:
Journal of Chinese Physician
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism of c-jun antisense gene transfection in protecting cardiomyocytes from injury of hypoxia and burn serum treatment. Methods Burn sera were collected from wistar rat with 30% total body surface area(TBSA) Ⅲ degree burn injury, and the mixture gas containning 1% O 2 was used as hypoxia model. The c-jun antisense gene recombinant vector was constructed. Neonatal wistar rat cardiomyocytes cultured in vitro were treated with hypoxia and burn sera. c-jun antisense gene recombinant vector was transfected into the cultured cardiomyocytes. Expressions of c-jun, PKC? and JNK were detected with western blot in the transfected and non-transfected cardiomyocytes. Results Expression levels of c-jun, PKC? and JNK significantly increased in the non-transfected cardiomyocytes when treated by hypoxia and burn sera, up to maximum 24 hour after the treatment. Expression levels of c-jun, PKC? and JNK in the transfected cardiomyocytes decreased significantly compared with non-transfected cells. Conclusions The transfection of the c-jun antisense gene recombinant vector protected cardiomyocytes from injury of hypoxia and burn sera treatment via down-regulating PKC? and JNK expressions.
