Detection of E-cadherin gene promoter hypermethylation in plasma of breast cancer patients
- VernacularTitle:乳腺癌患者血浆中E钙粘素基因启动子甲基化的检测与临床意义
- Author:
Jingjie ZHANG
;
Tao OUYAN
;
Guoren DENG
;
Wenhui WAN
;
Guangwei XU
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Methylation;
E-cadherin
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility in early diagnosis, predicting therapeutic effect, recurrence monitoring by examining promoter hypermethylation for cancer-associated genes E-cadherin in cancer tissue and peripheral blood of breast cancer patients. Methods The tumor tissue, paracancer- tissue and the paired plasma were examined for aberrant methylation of E-cadherin gene by methylation-specific PCR in 42 cases of breast cancer and 10 cases of breast benign diseases. Results The incidence of promoter hypermethylation of E-cadherin in tumor tissues was 52.4% and the paired plasma were 33.3%. E-cadherin hypermethylation in plasma samples and tumor samples significantly correlated with each other ( P
