Effect of paclitaxel on apoptosis and extracellular signal regulated kinase expression of cholangiocarcinoma RBE cell line
- VernacularTitle:紫杉醇对人胆管癌RBE细胞凋亡及细胞外信号调节激酶表达的影响
- Author:
Jun LI
;
Xueguang ZHU
;
Bo WANG
;
Hua ZHAO
;
Na ZHAO
- Publication Type:Journal Article
- Keywords:
Cholangiocarcinoma;
Cell death;
Protein kinases
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect on apoptosis and extracellular signal regulated kinase (ERK) expression of cholangiocarcinoma RBE cell line induced by paclitaxel and the role of extracellular signal regulated kinase in paclitaxel-induced apoptosis. Methods By techniques of cell culture in vitro, annexin-V-FITC and propidium iodide(PI)double staining and flow-cytometry (FCM), RBE cell line was treated by paclitaxel or together with MEK inhibitor PD98059 for 24 h. Using Western blot test the expression of ERK1, ERK2, pERK after RBE cell line was treated by paclitaxel or together with PD98059. Results Paclitaxel induced RBE cell line to apoptosis, the ratio of apoptosis was 14.8%?2.7%(n=6), the control apoptosis was 3.4%?1.3%(n=6,P
