Study on the Determination Method of Ornidazole in Mother's Milk by HPLC
- VernacularTitle:高效液相色谱法测定母乳中奥硝唑的方法学研究
- Author:
Dongmei YE
;
Yu PANG
;
Hongjian LIN
;
Pancai CHI
- Publication Type:Journal Article
- Keywords:
ornidazole;
HPLC;
mother's milk
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for determining the concentration of ornidazole in mother's milk METHODS:Stationary phase was Nova-Pak C8 column Mobile phase was methanol-water(30∶70) Samples were precipita_ted by acetonitrile without being extracted with organic solvent RESULTS:The endogenous substances in milk did not interfere with the determination of ornidazole The calibration curve was linear within the concentration range of 2 0～30 0?g/ml,r=0 9 997;the limit of detection was 0 49?g/ml;the average recovery was(99 67?0 54)%,and the within-day and between-day RSDs did not exceed 2 5% and 2 1%,respectively CONCLUSION:The method is simple,quick,accurate,sensitive,precise and usable for pharmacokinetic study of ornidazole in mother's milk
