Alteration of p38 MAPK activity in lung tissue in diabetic rats
- VernacularTitle:糖尿病大鼠肺组织p38 MAPK活性变化的研究
- Author:
Jingping ZHANG
;
Guolian LIU
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Lung;
Rats;
Transforming growth factor beta;
Protein kinase C;
Mitogen-activated protein kinases
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the pathologic changes in lung and the role of p38 MAPKinase signal pathways in pulmonary alteration in diabetic rats. METHODS: Diabetic rats were induced by intraperitoneally injected streptozotozin (STZ). After 4 weeks,we observed the pathologic changes in lungs,tested protein kinase C (PKC) activities by isotope in lungs of model rats,tested transforming growth factor (TGF-?_1) by Western blotting and immunohistochemical analysis,and determined the expression of p38 MAPKinase mRNA using in situ hybridization.RESULTS: After STZ administration for 4 weeks,we observed thickened pulmonary capillary basal lamina and increased number of fibre in Diabetes mellitus (DM) rats. TGF-?_1 levels,PKC and p38 MAPK activities were also found increased. CONCLUSION: The increased activities of TGF-?_1 and p38 MAPK suggeste that TGF-?_1 may play an important role in diabetic lung,and hyperglycemia-PKC-p38 MAPK signal pathways may be involved in the pathogenesis of diabetes.
