Influence of Active Macrophages on the Vascular Endothelial Biological Characteristics and the VEGF Receptor mRNA and ? ? ?_3 Expression
- VernacularTitle:活化巨噬细胞对血管内皮细胞生物学性状和VEGF受体、整和素???_3表达的影响
- Author:
Liang LIU
;
Xusheng LIU
;
Xiaoqi ZHANG
- Publication Type:Journal Article
- Keywords:
Macrophages;
Vascular endothelia;
Co-culture
- From:
Journal of Chinese Physician
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of active macrophages on the vascular endothelial participation in angiogenesis. Methods Vasculuar endothelial cell line ECV-304 was inoculated and cultured to 60% confluence.ECV-304 cells were then cultured lonely, with ConA(24?g/ml), with macrophage cell line U937(1?10 5/ml) or with U937 and ConA together to make four groups.The change of the ECV-304 cell cycle was determined by flow cytometry and that of the DNA synthesis was detected by 3H-TdR incorporation test. The change of the expression of endothelial VEGF receptor KDR mRNA was detected by RT-PCR while that of the integrin receptor ? ? ? 3 expression by immune fluorescent antibody technique on flow cytometry. Results Endothelial cells in S phase increased obviously with increased DNA synthesis in ECV-304 cells stimulated by active macrophages. In addition, the expression levels of the VEGF receptor KDR mRNA and integrin receptor ? ? ? 3 up-regulated significantly(P
