Inhibition of MHCⅡExpression on Human Dermal Fibroblast Through CⅡTA Anti-sense RNA
- VernacularTitle:CⅡTA反义RNA抑制皮肤成纤维细胞表面MHCⅡ类抗原表达
- Author:
Rong GUO
;
Ping ZOU
;
Huazhong LU
;
Huahua FAN
;
Yilin CAO
;
Lei CUI
;
Wei LIU
;
Qingxin SHANG
;
Bin ZHENG
;
Li GAO
;
Feng GAO
- Publication Type:Journal Article
- Keywords:
Gene expression regulation;
RNA,antisense;
Fibroblasts;
HLA-D antigens
- From:
Chinese Journal of Dermatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of using anti-sense RNA against classⅡmajor histocompatibility complex(MHCⅡ)transactivator(CⅡTA),which might regulate MHCⅡexpression,to suppress the relative immune response.Methods Stable transfectants of dermal fibroblasts with pDarⅡ(pDarⅡ-D)were tested for the expression of classic MHCⅡ(HLA-DR,-DP,-DQ)antigens induced with recombinant human interferon-gamma(IFN-?).mRNA abundance of CⅡTA,and classic MHCⅡwas mea-sured by RT-PCR.IL-2mRNA expressed in T cells,stimulated by transfected dermal fibroblasts,was de-termined by mixed lymphocyte reaction.Results When induced with IFN-?,the expression of HLA-DR and-DP antigens on pDarⅡ-D was reduced by95.63%and87.89%,respectively.Meanwhile,the mRNA contents of CⅡTA and classic MHCⅡwere decreased significantly(P
