The Mutations and Sequencing of HPV16E6E7Gene of Human Papillomavirus from Patients with HPV Infection in Beijing
- VernacularTitle:北京地区人乳头瘤病毒16 E6E7基因变异和序列分析
- Author:
Yagang ZUO
;
Jiabi WANG
;
Xuemei XU
;
Mingzhao ZHU
;
Fang LIU
;
Jingyi SI
;
Guoxing SONG
- Publication Type:Journal Article
- Keywords:
Papillomavirus,human;
Genes,viral;
E6E7gene;
Sequence analysis,DNA
- From:
Chinese Journal of Dermatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the gene sequence and mutations of human papillomavirus(HPV)type16E6E7in patients with HPV infection in Beijing.Methods Sample DNA was extracted from lesions in patients with HPV infection.HPV types were identified by polymerase chain reaction(PCR).E6E7gene,isolated from samples infected with HPV16only,was cloned into plasmid pGEM-3zf and sequenced.Results The recombinant plasmid pGEM/16E6E7was constructed successfully.The whole HPV E6E7gene was776bp in length which was equal to that of the standard strain.Three nucleotides exchanges,i.e.,p60PROE6,p96GLUE6,p565SERE7,were found in E6E7gene.Conclusion The data suggest that there are nucleotide differences of HPV E6E7gene between HPV obtained from Beijing and that of standard sequence.
