Cloning of p15~( INK4b) related gene CCRG and expressio n analysis in the renal carcinoma
- VernacularTitle:p15~(INK4b)相关基因CCRG的克隆及其在肾癌中的表达
- Author:
Guihua JIN
;
Xiongjun YE
;
Zhiji CHANG
- Publication Type:Journal Article
- Keywords:
Genes,p15;
Kidney neoplasms;
Gene amplification
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM and METHODS: To analysis the factor that involved in renal carcin ogenesis, we used the bait gene AK001518 to screen GenBank. To understand the re lationship between cell cycle related gene(CCRG) and p15, we did RT-PCR and Nort hern Blot experiments. Th en we examined CCRG expression level in renal carcinogenesis. RESULTS: Gained a function unknown gene CCRG that was 67% a mino acid identical with the gene AK00 1 518 that was regulated by p15. It was shown that the CCRG mRNA was dramatically d ecreased when p15 gene was over-expressed. CCRG expression level was much higher i n tumor tissues and cells than normal tissues and cells. CONCLUSION: The novel g ene CCRG expressed highly in the renal carcinoma, which might play a significant role in the renal carcinogenesis.
