Effect of hypoxia on the expression of proliferating cell nuclear antigen and phenotype of cardiac fibroblasts
- VernacularTitle:低氧对心脏成纤维细胞增殖细胞核抗原表达与表型的影响
- Author:
Jun YU
;
Miaozhang ZHU
;
Jianming PEI
;
Baoying CHEN
;
Shunyan L
;
Mingqing DONG
;
Qimin WEI
- Publication Type:Journal Article
- Keywords:
Anoxia;
Cardiac fibroblasts;
Proliferating cell nu clear antigen;
Phenotype;
Actins
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate effect of hypoxia on the expression of proliferating cell nuclear antigen(PCNA) a nd phenotype of cardiac fibroblasts(CFs). METHODS: The purif ied cardiac fibro blasts were cultured and divided randomly into there groups :control group, mode rate hypoxia(MH) group and severe hypoxia(SH) group. After 72 h,MTT method was u s ed to investigate the proliferation of CFs, and the ultrastructure of fibroblast s were observed with transmission electron microscopy The expression of PCNA a n d ?-actin in cardiac fibroblasts were measured by the means of immunohistochemi s try and laser scanning confocal microscopy, respectively. RESULTS: MTT A 490 nm value of MH group was significantly higher than that of control group by (18 4?25 0)% ( P
