Biological characteristics of long passaging rat mesenchymal st em cells
- VernacularTitle:长期传代的大鼠骨髓间质干细胞的生物学特性分析
- Author:
Junxia LEI
;
Haowei LI
;
Chunnong HUANG
;
Meiling ZHU
;
Guanmei WEN
;
Xiuming ZHANG
;
Yan LI
;
Shunon LI
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Mesenchymal stem cells;
Karyotyp ing;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate multi-potential of rat bo ne marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC w ere long passaged in vitro. METHODS: Cellular cycles of diff erent passages were assayed by FA CSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS: The early passages and long-term passages all showed st rong proliferation; passage 6, passage 25 and passage 45 all showed normal karyo type. CONCLUSION: Long-term culture and passage of rBMMSC still remain s strong proliferation. With this capability, the mutation inclination is not enhanced.
