Rat urotensin-II-induced main pulmonary artery contractions are mediated by MAPK *
- VernacularTitle:大鼠尾加压素Ⅱ收缩大鼠离体肺主动脉环与MAPK相关(英文)
- Author:
Shaoxian CHEN
;
Bicheng XUE
;
Yongsheng GONG
;
Xiaofang FAN
;
Lianggang HU
;
Yanfan CHEN
;
Liangxin WANG
- Publication Type:Journal Article
- Keywords:
Urotensins;
Mitogen-activated protein kinases;
Pulmonar y artery
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate rat Urotensin-II(ra t U-II)-induced vasoconstriction of rat main pulmonary arteries and the role of mitogen-activated protein kinase(MAPK). METHODS: The main pulmon ary artery was dissected from the male Sprague -Dawley rats and artery ring width was 3-4 mm. Concentration-response curves wer e gene rated to rat U-II(0 03 nmol/L-30 nmol/L).Inhibitor of MAPK,PD 98059(0 1 ?mol/ L -10 ?mol/L) were added into the medium after rat U-II(30 nmol/L)induced vasoc onstriction had reached plateau to construct the relaxant concentration-respons e curves and their EC 50 and E max . RESULTS: Rat U-II was a potent vasoconstrictor of isolated rat main pulmonary arteries [EC 50 =7 95?0 4 0, E max =(14 28?6 34)% of the response to 60 mmol/L KCl]; PD 98059 caused c oncentration-dependent relaxations of rat U-II precontracted arteries [EC 50 =5 91?0 45, E max =(81 39?13 65)%]. CONCLUSION: Rat U- II was a potent vasoconstrictor of rat main pulmonary arteries and this response was med iated through MAPK.
