Overexpression of neurokinin-2 receptor is related to pain in chronic pancreatitis
- VernacularTitle:神经激肽-2受体的过度表达与慢性胰腺炎的疼痛相关(英文)
- Author:
Xin SHI
;
Nairong GAO
;
Mingdong HUO
;
Yongjiu YANG
;
Haolin HU
;
Qingming GUO
;
Fries HELMUT
- Publication Type:Journal Article
- Keywords:
Pancreatitis;
Receptors, neurokinin-2;
Pa in
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of neurok inin-2 receptor (NK-2R) in normal pancreas and chronic pancreatitis (CP) tissues. The relation of expres sion of NK-2R with pain in CP was also evaluated. METHODS: CP tis sues were ob tained from 18 men and 7 women undergoing pancreatic head resection as a result of CP. Normal human pancreatic tissues were obtained from 20 patients (11 males/ 9 females). Real-time quantitative reverse transcription polymerase chain reacti on was used to determine the mRNA expression of NK-2R, Western blot analysis was used to determine its protein expression level, and immunohistochemistry was us ed to localize expression site of NK-2R protein. NK-2R mRNA level and pain were also analysed whether correlation exists. RESULTS: NK-2R mRNA and p rotein level were enhanced expressed in CP tissue samples compared with normal pancreas. Ove rexpression of NK-2R was related to the intensity ( r=0 59, P
