Effect of tea-polyphenols on the activation of NF-?B an d the expression of TGF-?_1 mRNA in THP-1 cells incubated with VLDL, LDL or ox-LDL
- VernacularTitle:茶多酚对高脂环境中THP-1细胞核因子-?B、转化生长因子-?_1 mRNA表达的影响
- Author:
Xiaogang ZHANG
;
Yunzhen CHEN
;
Han LEI
;
Zhoubi WANG
- Publication Type:Journal Article
- Keywords:
Tea-polyphenols;
NF-?B;
Transforming growth factor beta
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of tea-polyphe nols (TP) on the activation of NF-?B and the expression of TGF-? 1 mRNA in TH P -1 cells (a human acute monocytic leukemia cell line). METHODS: TH P-1 cells were incubated with the different concentrations of TP, VLDL, LDL or o x-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-?B was detected with immunohistochemistry technique, the positive index of the TGF-? 1 mRNA ex p ression was detected by hybridization in situ, and accumulation of total cholest erol (TC) in cells incubated with 0 4-40 ?g/L TP was determined with oxidase a ssay. RESULTS: The nuclear malposition rate of NF-?B, the posit ive index of the TGF-? 1 mRNA expression and TC in THP-1 cells incubated with 0 4-40 ?g/L of TP were lower than those with 0 ?g/L of TP in TP-V group, TP-L group and TP-O ( P 0.05). CONCLUSION: TP inhibited the activation of NF-?B, the expressio n of TGF-? 1 mRNA and the foam cell formation in the mono-macrophage.
