Proliferative activity for a cholangiocarcinoma cell line of bile from patients with choledocholithiasis
- VernacularTitle:胆管结石患者胆汁对人胆管癌细胞增殖的影响
- Author:
Gaosong WU
;
Zhengren LIU
;
Shengquan ZOU
;
Fazu QIU
- Publication Type:Journal Article
- Keywords:
Cholelithiasis;
Bile;
KG1Biliary treat neoplasmsHJHK
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of bile from patients with choledocholithiasis on the growth of human cholangiocarcinoma cell line QBC939 and the potential relation between choledocholithiasis and cholangiocarcinoma. MethodsWT5”BZ Choledocholithiasis bile (CB) and normal bile (NB) specimen were used for this study. The proliferative effects of bile were measured by methabenzthiazuron (MTT) assay and cell cycle and apoptosis were analyzed by flow cytometry. KG2Results CB significantly promoted the proliferation of QBC939 cells, QBC939 proliferative index increased significantly after treated with 1% CB for 48 h ( P
