Effects of mutant tPA gene transfer on tPA activity and adherence of endothelial cells seeded onto graft vessel
- VernacularTitle:转基因血管内皮细胞的组织型纤溶酶原激活剂活性和种植黏附性研究
- Author:
Li CHEN
;
Ning DAI
;
Hong YU
;
Sifeng TAO
;
Jianwei WANG
;
Bin XU
- Publication Type:Journal Article
- Keywords:
Endothelium, vascular;
Plasminogen activators;
Genes;
Blood vessel prosthesisHJHK
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the activity of tissue plasminogen activator (tPA) gene transduced endothelial cells (EC) and the cell retention on graft vessel. MethodsKG1 EC were transduced with pseudotyped vectors carrying genes coding for either the wild type tPA or mutant tPA. The supernatants were collecteJPd and assayed for tPA activity in the presence and absence of fibrin. Polytetrafluoroethylene (PTFE) vessel grafts were seeded with EC, and then exposed to an in vitro flow system for 1 h. The number of EC on grafts were counted and the retention of EC were evaluated. ResultsWT5”BZ The tPA activity of the nontransduced EC was (1 5?1 0) IU/ml, while that of wild type tPA gene transduced EC increased to (30.0?8 0) IU/ml, mutant type tPA gene transduced EC increased to (14.1?1 0) IU/ml( P
