The detection of c-myc,Bcl-2 protein and DNA ploidy in invasive breast carcinoma and its significance
- VernacularTitle:浸润性乳腺癌中c-myc,Bcl-2蛋白表达及DNA倍性的检测及意义
- Author:
Dong ZHANG
;
Dongchen GAO
;
Changhuai ZHANG
;
Yu WANG
;
Xiaomei LU
;
Shuyuan CHEN
;
Yan LU
- Publication Type:Journal Article
- Keywords:
Breast neoplasm;
Genes, c-myc;
Bcl-2;
Immunohistochemistry;
Flow cytometryJPHJHK
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical significance of c-myc、Bcl-2 protein expression, DNA ploidy and their relationship in breast cancer. MethodsWT5”BZ The expression of c-myc、Bcl-2 protein in 146 breast tumor tissues was examined by using immunohistochemical methods(S-P)and DNA ploidy in 72 cases of breast carcinoma by flow cytometry. ResultsKG1 Nuclear expression of c-myc protein was detected in 9 6% of tumors, and it was related to the DNA aneuploidy and prognosis. Cytoplasmic expression of c-myc protein was present in 91 8% of the carcinomas. Moderate to strong c-myc protein expression in cytoplasmic was associated with the positive status of ER. Bcl-2 protein was positive in 78 8% of cancers. Overexpression of Bcl-2 protein was associated with ER(+) status and the lack of axillary lymph node metastasis. DNA aneuploidy was found in 51 4% of 72 cancers, it was related to nuclear expression of c-myc protein and prognosis.WT5”HZConclusion Nuclear expression of c-myc protein and DNA ploidy are important prognostic factors.
