The Effects of hTERT Transfection on the Collagen Expression of Human Embryonic Fibroblasts
- VernacularTitle:人端粒酶催化亚单位基因转染人胚胎成纤维细胞后胶原的变化
- Author:
Guangping LIANG
;
Xiangdong LUO
;
Yongyue SU
- Publication Type:Journal Article
- Keywords:
hTERT;
Human Embryonic Fibroblast;
Collagen
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The recombinant fluorescent eukaryotic expressing vector containing hTERT cDNA was transfected into human embryonic fibroblasts (hEFs) to explore the effects of exogenous hTERT on the type I and III collagens expression in hEFs. Methods p IRES2-EGFP-hTERT plasmid and pIRES2-EGFP plasmids were transfected into primary hEFs respectively by Lipofectin reagent. Expression of type I and III collagen was determined by Western blotting and the content of type I and III collagens in the cellular medium at 3 days after transfection were examined by radio-immunoassay. Results The expression levels of type I and III collagens in hTERT gene transfected hEFs(hEF-hTERT) were obviously higher than those in untransfected hEFs and vacant vector transfected hEFs(hEF-EGFP). The content of type I and III collagens in the cellular medium in hEF-hTERT cells at 3 days after transfection was also higher than that in untransfected hEFs and hEF-EGFP cells. Conclusions The synthesis ability of type I and III collagens in hEFs could be promoted by exogenous hTERT gene transfection.
