Genetical Engineering of Humanized Anti-keratin Antibodies with High Affinity
- VernacularTitle:高亲和力抗角蛋白人抗体的基因工程制备
- Author:
Gang WANG
;
Yan WANG
;
Yufeng LIU
- Publication Type:Journal Article
- Keywords:
Keratin;
Antibodies, monoclonal;
Antibody affinity;
Gene expression
- From:
Chinese Journal of Dermatology
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To produce humanized anti-keratin antibodies with high affinity. Methods Panning of phage antibody libraries against human epidermal keratin was conducted to select specific antibodies. Random mutation method and chain-shuffling technique for the gene of the antibodies were employed to improve antibody affinity. The binding activity, specificity and affinity of the obtained antibodies were de-termined and analyzed. Results Four clones of either IgG or IgM anti-keratin antibodies were seleeted from semisynthetic or natural antibody libraries. One of them was seleeted to undergo affinity maturation. Random mutation method for the gene did not result in any improvement in antibody affinity. By chain-shuffling technique, however, affinity level was markedly increased and an antibody with affinity of 8?1010/M was isolated. Conclusion It is feasible to generate humanized antikeratin antibodies using genetical engineering technique.
