Upregulation of proto-oncogene c-mpl by interleukin-13 in Dami cells
- VernacularTitle:白细胞介素-13上调巨核细胞系-Dami细胞原癌基因c-mpl的研究
- Author:
Keqiang LI
;
Wenlin LI
;
Xiaoyu SHI
;
Shuyou PENG
;
Yingbin LIU
;
Hon LIU
- Publication Type:Journal Article
- Keywords:
Interleukin-13, recombinant;
Dami cell;
Proto-Oncogene c-mpl;
Thrombopoietin
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of recombinant human interleukin-13 (rhIL-13) on the expression of proto-oncogene c-mpl in Dami cells, a human megakaryobiastic leukemia cell line. METHODS: The expression of c-mpl mRNA in Dami cells was investigated with RT-PCR. The expression of membrane-bound protein c-mpl on Dami cells was investigated with ligand combination experiment. RESULTS: In RT-PCR experiment, we found the quantitis of expression of c-mpl mRNA in 25 ?g/L rhIL-13 group increased by 24.8%. In ligand combination experiment, we found quantitis of expression of membrane-bound protein c-mpl in 100 ?g/L rhIL-13 group increased by 28.5% ( P
