Changes of nitric oxide synthase and nitric oxide in lung of smoking rats
- VernacularTitle:吸烟大鼠一氧化氮合酶和一氧化氮的变化
- Author:
Danlei YANG
;
Yongjian XU
;
Zhenxiang ZHANG
;
Chaoqian LI
- Publication Type:Journal Article
- Keywords:
Nitric-oxide;
Nitric-oxide synthase;
Smoke;
Inflammation
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the changes of iNOS and eNOS in lung tissue and NO in bronchoalveolar lavage fluid (BALF) in smoking rats. METHODS: 80 Wistar rats were divided into control, smoking group, L-NIL group and L-NAME group (rats were exposed to smoke and injected (i.p.) with selective iNOS inhibitor L-NIL or NOS inhibitor L-NAME). iNOS and eNOS protein levels in whole lung were detected by immunohistochemical staining, and NOS mRNA was quantified using RT-PCR. In addition, NO - 2/NO - 3 was determined using Griess assay. RESULTS: The expression of iNOS mRNA and protein in smoking rats increased, the expression of eNOS mRNA and eNOS protein decreased, and the total cell count and the level of NO - 2/NO - 3in BALF increased( P
