Effect of cocaine on caspase-3 in myocardiac cells of male rats in different age
- VernacularTitle:可卡因对不同年龄段大鼠心肌细胞caspase-3活性的影响
- Author:
Hongju WANG
;
Yuanwei HUANG
;
Guosheng YANG
;
Le ZHANG
- Publication Type:Journal Article
- Keywords:
Myocardium;
Cells;
Apoptosis;
Caspases;
Flow cytometry;
Cocaine
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of cocaine on caspase-3 in myocardiac cells of male rats in different age. METHODS: Three-week-old( n= 16), six-week-old( n= 16) and twelve-week-old( n= 16)male Sprague Dawley rats were all divided into control groups and experiment groups randomly, each group had eight animals, experiment groups were given cocaine hydrochloride (15 mg?kg -1 body weight) subcutaneously daily for four weeks.The ratio of heart weight to body weight (HW/BW,mg/g) were measured. DNA fragmentation of myocardia cells was determined by gel electrophoresis, and caspase-3 activity in myocardia cells was tested by flow cytometry (FCM). RESULTS: In three experiment groups, the DNA isolated from myocardial cells displayed clear ladder pattern. The HW/BW and the caspase-3 activity were increased significantly than those of control groups ( P
