Detection of Glu-plasminogen in the human plasma and its clinical significance
- VernacularTitle:人血浆谷氨酸型纤溶酶原的检测及其临床意义
- Author:
Dao LI
;
Quan CHEN
;
Hong WANG
;
Jianxin LI
;
Hongl WANG
- Publication Type:Journal Article
- Keywords:
Surgical procedures, operative;
Plasminogen;
Antibodies, monoclonal;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the changes of plasma content of Glu-plasminogen (plasmin, Pln) in patients during blood coagulation and/or fibrinolytic system activation. METHODS: Using specific McAb to antigenic determinant in NH 2 terminal (1-65 aa) of Pln and specific McAb to antigenic determinant in heavy chain of plasminogen, the sandwiched ELISA method was established to detect Glu-plasminogen and total plasminogen in human plasma collected from 220 normal controls and 40 patients after heart surgery. RESULTS: The average total plasminogen was (231.8?62.1) mg/L and average Glu-plasminogen was (231.9?45.8) mg/L in 220 normal controls, the ratio of Glu-plasminogen to total plasminogen (G/P) was 0.91. The ratio of Glu-plasminogen [(152.4?68.1) mg/L] to total plasminogen [(268.9?73.3) mg/L] in 40 patients after heart surgery was significantly lower than that in normal controls ( P
