Expression of peroxisome proliferator-activated receptor ? in rat fatty liver
- VernacularTitle:过氧化物酶体增殖物激活受体?在实验性大鼠脂肪肝中的表达
- Author:
Xiaohong QI
;
Zhaoping ZHANG
;
Xiaoyu LI
;
Lingli QUE
;
Cuizhe WU
- Publication Type:Journal Article
- Keywords:
Rats;
Fatty liver;
Peroxisome proliferator;
Tumor necrosis factor;
Fatty acids, nonesterified
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of expression of peroxisome proliferator-activated receptor ?(PPAR ?) in pathogenesis of rat fatty liver.METHODS: The rats were treated with a low dose of carbon terachloride (CCl 4) and fed a high fat diet to produce fatty liver. We determined the concentrations of triglyceride (TG), total cholesterol (TC), free fatty acid (FFA) in liver and the alanine aminotransferase (ALT) activity, tumor necrosis factor-? (TNF-?), FFA in serum and the degree of hepatocytic steatosis. Total RNA of liver was extracted, and the expression of PPAR ? were analyzed by semi-quantitative RT-PCR method. RESULTS: In model group, the hepatocytic PPAR ? mRNA expression decreased to 0 41?0 28, compared to 1 41?0 29 in the control group ( P
