Effects of hypoxia and hypercapnia on expression of soluble guanylate cyclase in rat pulmonary artery
- VernacularTitle:低O_2高CO_2对大鼠肺中小动脉可溶性鸟苷酸环化酶基因及其蛋白表达的影响
- Author:
Xiaodong XIA
;
Zhengjie XU
;
Qunji WANG
;
Hongqi ZHANG
- Publication Type:Journal Article
- Keywords:
Hypoxia;
Hypercapnia;
Pulmonary artery;
Soluble guanylate cyclase;
Genes
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of soluble guanylate cyclase protein and its mRNA in rat pulmonary artery after exposure to hypoxia and hypercapnia. METHODS: Male Sprague-Dawley rats were randomly split into 4 group, which were hypoxic hypercapnic (HH 1 week, HH 2 weeks, HH 4 weeks) group and control group, to copy pulmonary hypertensive animal model. The expression of sGC? 1 and ? 1 subunits protein of medial and small pulmonary artery was performed by immunohistochemistry with a polycolonal antibody. In situ hybridization was performed on the rat lung tissue using sGC oligonuclear probe to assay the expression of sGC? 1 subunit mRNA. RESULTS: The sGC? 1 and ? 1 subunits protein and sGC? 1 subunit mRNA were faint staining in the pulmonary small and medium artery in HH1 week, HH 2 weeks and HH 4 weeks groups compared with control group (all P
