In situ detection and significance of hTERT in giant cell tumor of bone
- VernacularTitle:骨巨细胞瘤组织中端粒酶活性的原位检测及意义
- Author:
Huiyong SHEN
;
Yong TANG
;
Shangli LIU
;
Haigan LI
- Publication Type:Journal Article
- Keywords:
Giant cell tumor of bone;
Telomerase;
In situ hybridzation
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase, and hTERT mRNA expression contributes to telomerase activity. We therefore assessed the significance of hTERT expression in the biological behavior of giant cell tumor(GCT) of bone. METHODS: In situ hybridization was used to identify the expression of hTERT mRNA in 27 formalin-fixed paraffin-embedded clinical specimens from human GCT. RESULTS: 8 cases (8/27)displayed positive hTERT mRNA signal, positive hTERT mRNA signal located in the cytoplasms of mononuclear stromal cells and some multinuclear giant cells. CONCLUSION: The expression of telomerase in paraffin-embedded tissue can be evaluated efficiently and effectively by in situ hybridization, and the results suggest that there may be a neoplastic multinuclear giant cell type in human GCT.
