Effects of Bene Jones protein and TGF-?_1 on the proliferation of rat renal proximal tubular cells in vitro
- VernacularTitle:尿本周氏蛋白及转化生长因子?_1对肾近端小管上皮细胞增殖的影响
- Author:
Zhenhai ZHOU
;
Youji LI
;
Yunxian CHEN
;
Xiaoying LI
;
Xueqing YU
;
Juan LI
;
Shaokai LUO
;
Yaoguan YUAN
- Publication Type:Journal Article
- Keywords:
Bence Jones protein;
Transforming growth factor beta;
Renal proximal tubular cell
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of Bene Jones protein (BJP) from multiple myeloma(MM) patient and TGF-? 1 on cultured renal proximal tubular cell(PTC) proliferation. METHODS:[ 3H] TdR incorporation was used to study the effect of ?BJP and TGF-? 1 on cultured rat NRK.52E PTC proliferation,the expression of TGF-? 1 in the supernatant of PTC cultured with BJP was assessed with ELISA. RESULTS:① [ 3H] TdR incorporation of PTC was inhibited by BJP in a dose-dependent manner,when co-cultured with 100-800 ?mol/L BJP and 2.0 ?g/L TGF-? 1, the [ 3H] TdR incorporation was lower than that of BJP alone, especially when BJP≥400 ?mol/L; ②The expression of TGF-? 1 in the supernatant of PTC cultured with BJP was increased ,especially when BJP≥400 ?mol/L( P
