Changes in expression of TNF-a and IL-8 mRNA in skeletal muscle induced by cardiopulmonary bypass during cardiac surgery
- VernacularTitle:体外循环心脏手术中骨骼肌TNF-?和IL-8 mRNA表达的变化
- Author:
Feilong HEI
;
Qiang HU
;
Qing GONG
- Publication Type:Journal Article
- Keywords:
Cardiopulmonary bypass;
Cardiac surgical procedures;
Muscle, skeletal;
Interleukin-8;
Tumor necrosis factor
- From:
Chinese Journal of Anesthesiology
1996;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in tumor necrosis factor-alpha ( TNF-a) and interleukin-8 (IL-8) mRNA in skeletal muscle during open heart surgery with cardiopulmonary bypass (CPB) . Methods Ten patients (4 male, 6 female) aged 35-62 yr undergoing valve replacement under CPB were enrolled in this study. Anesthesia was induced with midazolam, fentanyl and pancuronium and maintained with isoflurane-fentanyl and pancuronium. A specimen of rectus muscle of abdomen (2g) was taken while chest was being opened (baseline) and closed. Blood samples were obtained before anesthesia (T0), while chest was being opened (T1), 15 min after initiation of CPB (T2), 5 min after aortic unclampmg (T3) and while chest was being closed (T4) for determination of plasma TNF-a and IL-8 level using enzyme-linked immunosorbent assay technique. Results TNF-a and IL-8 mRNA expression in skeletal muscle were significantly increased while chest was being closed as compared with the baseline ( P
