Expression and significance of RhoC gene in primary hepatocellular carcinoma
- VernacularTitle:RhoC基因在肝细胞癌中的表达及其意义
- Author:
Wei WANG
;
Lianyue YANG
;
Zhili YANG
;
Gengwen HUANG
;
Weiqun LU
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Genes;
Rho factor
- From:
Chinese Journal of General Surgery
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression of RhoC gene in primary hepatocellular carcinoma and to evaluate the relationship between RhoC gene expression and invasion and metastasis of primary hepatocellular carcinoma.MethodsThe mRNA expression of RhoC gene was examined by polymerase chain reation after reverse transcription (RT-PCR) in 25 cases of primary hepatocellular carcinoma (HCC) and adjacent non-cancerous tissuse. In addition, the mutation of RhoC gene was examined by polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP)ResultsThe mRNA expression of RhoC in tumor tissue were higher than that in adjacent liver tissue,1.8?1.1 vs. 1.0?0.7( P
