Analysis on Prenantal Fetomaternal Immuno-state of 32 RhD-negative Pregnant Women
- VernacularTitle:32例RhD阴性孕妇产前胎-母免疫状态分析
- Author:
Wen XIONG
;
Chaopeng SHAO
;
Lianghua WANG
- Publication Type:Journal Article
- Keywords:
Rh-Hr blood-group system;
Fetomaternal transfusion;
Erythroblastosis, fetal;
Rh isoimmunization
- From:
Chinese Journal of Perinatal Medicine
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Study on the relations between Rh hemolytic disease of the newborn (HDN) and the influencing factors of producing anti-D. Methods D antigens of 32 RhD-negative pregnant women and their newborns are determined by indirect antiglobulin test (IAT) and absorption/elution test. With polymerase chain reaction (PCR) and direct genomic DNA sequencing, we detect the RHD gene in weak D pregnant women identified serologically, and we analyzed the situation of fetomaternal hemorrhage (FMH) of the D-negative women with more than 2 gestations with flow cytometry. Results Among 32 pregnant women of RhD-negative detected by first test, there are 18 pregnant women with two and more pregnancy. In these 18 pregnant women, 3 cases are identified as D el phenotype, 1 case is designated as D category VI type III, the rest 14 cases are truly D-negative pregnant women. Among the truly D-negative multi-pregnant women, 2 produce anti-D in sera and 13 are detected fetal erythrocytes in their peripheral blood by flow cytometry. However there are no anti-D detected in sera of D-negative first-pregnant women. Conclusion No anti-D allo-immune response were observed in all first-time pregnant women. In multi-pregnant women, however, 14.3% produce anti-D and result in HDN of Rh.
