Continuing Activation of Caspase-3 Following Neonatal Rat Hypoxic-ischemic Brain Damage
- VernacularTitle:Caspase-3持续激活时程与新生大鼠缺氧缺血性脑损伤
- Author:
Juli WANG
;
Hongtao CAO
;
Yukun HAN
- Publication Type:Journal Article
- Keywords:
Hypoxic-ischemia, brain;
Caspases-3;
Apoptosis;
Animals, newborm;
Rats
- From:
Chinese Journal of Perinatal Medicine
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of caspase-3 activation and DNA fragmentation in later period of neonatal rat hypoxic-ischemic brain damage(HIBD). Methods DNA fragmentation,caspase-3 mRNA and caspase-3 protein were measured in 2 wks、3 wks and 4wks after setting HIBD animal model in newborn wistar rats by FCM, RT-PCR and Immunohistochemistry. Results (1) Apoptosis lasted 4ks after HIBD. This suggested a long lasting role of apoptosis in neonatal HIBD by TNNEL and EM.(2)Caspase-3 mRNA expression was estimated by semi-quantitative RT-PCR. It was higher in HIBD group(0.771?0.074) than in control group(0.620?0.038, P0.05. Average Avalue of Caspase-3 protein in HIBD group(0.374 ?0.038) at 3 wks was significantly higher than that in control group(0.356?0.020,P
