Temporal Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Preterm Rat Lungs Exposed to Hyperoxia
- VernacularTitle:基质金属蛋白酶及其抑制剂在高氧暴露下的早产大鼠肺组织中的动态表达
- Author:
Liling QIAN
;
Liwen CHANG
;
Zhihui RONG
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinases;
Tissue inhibitor of metalloproteinases;
Hyperoxia;
Lung;
Infent, preterm
- From:
Chinese Journal of Perinatal Medicine
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in hyperoxia-induced lung injury in preterm rats. Methods At the 2 nd postnatal day Sprague-Dawley preterm rats were randomly assigned to air group and hyperoxia group (exposed to about 85% of O 2). At 3,7,14 and 21 days after exposure, six rats of each group were used to assess lung histologic changes and expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in lungs by immunohistochemistry. At 3,7 and 14 days after exposure, gelatinase activity in bronchoalveolar lavage fluid (BALF) of another six rats in each group by gelatin zymography was examed. Results Except 3 d after exposure, hyperoxia group showed lung injury characterized by subacute alveolitis and inhibition of lung development. Expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in hyperoxia group was stronger than that in air group at every time (P
