The Effect and Significance of Intestinal Trefoil Factor IL-8 and MDA for Neonatal Rat Model for Hypoxia-induced Intestinal Injury
- VernacularTitle:肠三叶因子对新生鼠缺氧肠损伤模型白细胞介素8、丙二醛的影响及意义
- Author:
Liping CHEN
;
Binghong ZHANG
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Growth substances;
Peptides;
Enterocolitis, necrotizing;
Interleukin-8;
Malondiadehyde
- From:
Chinese Journal of Perinatal Medicine
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of intestinal trefoil factor (ITF) on interleukin-8(IL-8) and malondiadehyde (MDA) of neonatalrats' intestinal tissue post necrotizing enterocolitis(NEC), to investigate whether ITF has some protective effects on NEC.. Methods Thirty-two neonatal tats of NEC model were devided into four groups, (1)NEC+FTF 0.5 mg, (2) NEC+ITF 0.2 mg, (3) NEC, and (4) all others. After NEC model of neonatal rats was established and the ITF was treated, all the neonatal rats were returned to their mothers. In the 4th day, all the subjects were put to death. Intestinal tissue located at the boundary of ileum and cecum was obtained to observe hostological changes. Other intestinal sissue was removed to homogenate. Than the homogenate was centrifuged, supernates were used to determine the content of IL-8 and MDA. Results Content of IL-8 was dramatically less in group A and B than group C, respectively (29.722?7.134)、(30.512? 8.230)、(39.379 5?4.420) [pg/(mg?pro)](P0.05). Content of MDA was dramatically less in group A and B than group C, respectively(2.267?0.267)、(2.154?0.301)、(3.378?0.835)[nmol/(mg?pro)](P
