EGFR Activates AP-1 Through ERK-1/2 Signaling Pathway in the Rat Gastric Mucosal Cells
- VernacularTitle:大鼠胃黏膜细胞EGFR通过ERK-1/2信号传导通路激活AP-1
- Author:
Jianling LI
;
Hong YI
;
Xueping FENG
- Publication Type:Journal Article
- Keywords:
Gastric, mucosal, cells;
EGFR;
AP-1;
ERK-1/2;
Signaling, pathway
- From:
Journal of Chinese Physician
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the signaling pathway of AP-1 activated by epidermal growth foctor receptor(EGFR) in the rat gastric mucosal cells. Methods TGF-? stimulated freshly isolated rat gastric mucosal cells,and Western blot and EMSA detected the activation of ERK-1/2 signaling pathway. Results The exposure of the mucosal cells to 1 nmol/L TGF-? for 30 min significantly induced transcriptional activity of AP-1. This induction was associated with a concomitant activation of MEK and ERK-1/2. TGF-?-induced activation of AP-1 in gastric mucosal cells could be totally abrogated by either PD153035, a specific inhibitor of EGFR tyrosine kinase, or PD98059, a specific inhibitor of MEK. Conclusions Our findings suggest that EGFR AP-1 activates by EPK-1/2 signaling pathway in the rat gastric mucosal cells.
