Rapid Genomic DNA Fragments Extraction From Serum Denatured With High Temperature
- VernacularTitle:通过高温变性快速提抽DNA的方法学研究
- Author:
Yu WANG
;
Shu WEN
;
Xiaoyan LONG
- Publication Type:Journal Article
- Keywords:
Degeneration;
Serum;
DNA extraction;
Polymerase chain reaction
- From:
Journal of Chinese Physician
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an approach for a rapid extraction of genomic DNA fragments from serum.Methods Serum specimen from human was processed using high temperature denaturation and was centrifuged, then using various primer sets to amplify the DNA fragment extracted, so as to verify the efficiency of this DNA extraction method.Results The templates preparing from this DNA extraction method were able to guarantee the amplification of fragments shorter than 400 base pairs.Conclusion This method is simple, rapid and inexpensive.These genomic DNA fragment extracted can be utilized to amplify definite DNA template for polymerase chain reaction.
