Expression of Cytokeratin 19 in Different Live Tissues From Victims With Third-degree Burns in Repair Process
- VernacularTitle:角蛋白19在Ⅲ度烧伤创面修复过程中的表达及意义
- Author:
Pengxiang ZHOU
;
Chengqun LUO
;
Yonghong GU
- Publication Type:Journal Article
- Keywords:
Burns;
Epidermal stem cell;
Cytokeratin 19
- From:
Journal of Chinese Physician
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression characteristics of cytokeratin 19(CK19) in different live tissues from victims with third-degree burns to explore the possible mechanisms of tissue reparation and regeneration in orthophoria.Methods 10 young victims with third-degree burns were included in this study.Alive tissues at different regeneration stages were studied by immunohistochemistry with streptavidin-biotin-peroxidase method using specific anti-CK19 monoclonal antibody. Only cytoplasm expression was considered as specific.Normal skin tissue and chronic skin ulcer were used as controls.The study was focused on the distribution and morphological features of CK19-positive cells.Results ⑴In normal skin ,CK19-positive cells were seen in basialis layer of epiderm and cutaneous appendages.⑵CK19-positive cells were not found in subcutaneous tissue at 3～6 days after the injury,but they were seen in granulation tissues.⑶A lot of CK19-positive cells were detected in early regeneration tissues.⑷In late regeneration tissues,CK19-positive cells were detected in basialis layer of epiderm and cutaneous appendages.⑸Advanced regenerationed epidermal tissue was similar to that of normal.⑹No CK19-positive cells were observed in all of 6 cases with chronic skin ulcer.Conclusion It is possible that victims with third-degree burns could be healed by tissue regeneration orthophoria.
